Typically heterozygous rats are cryo-recovered in 10-12 weeks to an age of 7 weeks old. Subsequently homozygous rats to an age of 7 weeks old can be produced within 20-22 weeks.

• 29 bp deletion within Exon 2 on chromosome 3
• Homozygous Rag1 knockout rats display loss of RAG1 protein via Western blot
• Homozygous Rag1 knockout rats show loss of B and T cells by FACS analysis
• Background Strain: Sprague-Dawley

Availability: Cryopreserved as heterozygous embryos
Zygosity: Homozygous as live colony

Recombination Activation Genes (Rag) encode enzymes that play an important role in the rearrangement and recombination of the genes of immunoglobulin and T cell receptor molecules during the process of V(D)J recombination. Rag1 knockout rats lack mature B and T lymphocytes.

Mature B and T cells are critical components for an adaptive immune system. Rats deficient in Rag1 protein produce no mature B or T cells. This non-leaky model for severe combined immune deficiency is useful for vaccine development, as well as the study of autoimmune and infectious diseases.