Prkdc knockout rats: HsdSage:SD-Prkdcem1Sage GEM rats

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Cryopreserved

Typically heterozygous rats are cryo-recovered in 10-12 weeks to an age of 7 weeks old. Subsequently homozygous rats to an age of 7 weeks old can be produced within 20-22 weeks.

Background strain: Sprague Dawley
Homozygous knockout rats display total loss of protein via Western blot

Availability: Cryopreserved as heterozygous embryos

The Prkdc gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). DNA-PK is required for the non-homologous end joining (NHEJ) pathway of DNA repair, which rejoins double-strand breaks. It is also required for V(D)J recombination, a process that utilizes NHEJ to promote immune system diversity. Mature B and T cells are critical components for an adaptive immune system. DNA-PK knockout rats have severe combined immunodeficiency (SCID) and lack of both B and T cells, due to their V(D)J recombination defect. Rats deficient in the Prkdc gene produce no mature B, T or NK cells. This SCID rat is a useful model for cancer, xenografts, vaccine development, and autoimmune and infectious disease study.

Origin: The Prkdc model was originally created at SAGE Labs, Inc. in St. Louis, MO and distributed out of the Boyertown, PA facility. The line continues to be maintained through the original SAGE Labs animal inventory acquired by Envigo, then Envigo was acquired by Inotiv in 2021.

Available regions:

Asia-Pacific Austria Baltic states Belgium East Europe France Germany Ireland Italy Portugal Scandinavia Spain Switzerland The Netherlands United States United Kingdom Other regions

For pricing please contact us using the phone number above