Monoclonal Antibody Production

It is hard to imagine a world without monoclonal antibodies (mAbs), as reagents and therapeutics in their own right; they have played an important role in the advancement of basic research, diagnostics and product development.

The production of custom antigen specific mouse monoclonal antibody is a time consuming and technically demanding process. The production of antibodies is conducted at our state-of-the-art facilities either in the US or UK, utilizing Inotiv purpose bred models. Our highly-trained personnel provide you with unparalleled expertise across a wide range of antibody services.

In vivo (ascites) production

For an economical approach, Inotiv Bioproducts offers monoclonal antibody production as ascites in our FDA registered cGMP compliant facility. Our ready access to large numbers of high quality, genetically defined adventitious virus-free mice and rats ensures a rapid turn-around time at a competitive price. We can also help maximize your yield time through our innovative Optimization Program.

Service	Description	Cell line	Production type	Capacity	Advantages
Optimization program	Different doses tested Ascites available can be evaluated before use; Small to large run sizes	Hybridomas, NSO and SP2/0	Balb/c, Nude, SCID, and other species	Small to largeScale(10mg-100g)	Determine optimal cell dose; Allows maximal yield; Verifies specifications; Saves time
Research ascites production (non-cGMP)	When cGMP is not needed; Unique service; Same phases as cGM	Hybridomas, NSO and SP2/0	Balb/c, Nude, SCID, and other species	Small to large Scale (10mg-100g)	No MAP Testing requirement; Quicker turnaround time; Custom protocols; Reduced cost
cGMP production	Diagnostic/Therapeutic use; Extensive quality; Program fully tested	Hybridomas, NSO and SP2/0	Balb/c, Nude, SCID, and other species	Small to large Scale (10mg-100g)	AAALAC accredited cGMP compliant; Optimized production; Large animal inventory; Complete documentation

In vitro monoclonal antibody production

Inotiv Bioproducts has the expertise to produce your monoclonal or secreted protein in vitro. We have the capacity to produce 10mg-300g of research or cGMP antibody, or other secreted proteins, in our innovative tissue culture bag and hollow fiber production units. We offer a variety of media options such as RPMI, DMEM, IMDM, serum free, protein free, or other custom media formulations.

Service	Description	Cell line	Production type	Capacity	Advantages
Small scale production	Cost effective for smaller research runs Good for early feasibility, clonal selection and media optimization	Hybridomas (CHO’s, 293’s, PerC.6 and secreted proteins)	Tissue; Culture Bag; Hollow Fiber; Bioreactor	Up to 1 gram per project	Reduced cost Yield~20-150mg per 1 L bag; Quick turn-around; Excellent oxygen transport
Large scale production	Flexible and easy scale up; Larger scale research and cGMP runs	Hybridomas (CHO’s, 293’s, PerC.6 and secreted proteins)	Hollow; Fiber;Bioreactor	10mg-300g per project	Max environmental control; Reproducible manufacturing process; Reduces extraneous proteins; Highly concentrated product

Hybridoma development

Inotiv scientists, veterinarians and technicians have more than 40 years of experience refining our approach to developing monoclonal antibodies from a wide variety of peptides and proteins. While our procedures are well-defined, we are flexible when we need to be.

Inotiv Bioproducts' hybridoma development program is a flexible, interactive, and cost effective multi-phased service. We will work closely with you to design a program that will meet your requirements. Our custom development options include providing up to three, five, or ten clones, or as many as you require. As our client, you retain all rights to any clones that are produced. Once the clones are developed, we can scale them up as tissue culture supernatant.

Service	Requirements	Project outcomes
Stage I: Immunization	Immunize mice with antigen; Boost/test by ELISA or alternate method; Additional boost(s) if needed; Pre-fusion boost	Move mice with best titers as fusion candidates; Immunizing multiple mice increases success
Stage II: Fusion/screen and select	Fusion with myeloma cell line; Grow cells in HAT mediumInitial screen/selection; Screen	Screen all initial plates; Expand positive wells and test by ELISA; Set up to 30 positives for cloning; Keep frozen back–up for future testing
Stage III: Cloning/frozen stock	First cloning; Final cloning; Selection for non-HT requirement; Frozen stock and small antibody scale-up	Clone by limiting dilution method; Test clones by ELISA; Several cloning cycles; Final clones expanded and small amount of antibody produced

Customer experiences

When you work with Inotiv, you can be confident that through our experience, we ensure consistent and reliable products for research and industry.

Discover how Inotiv helps companies in providing custom antibody services, click here to read more.

Get in touch — Contact our antibodies and bioproducts team

At Inotiv, we are dedicated to helping you secure the potential of your product by providing you with a comprehensive selection of research animals. Our experts are ready to assist you.

If you have a more general inquiry click here to get in touch — general inquiry form.

North America
- Tel: +1 800 972 4362
- Bioproducts / antibodies: bioproducts.na@inotiv.com
Asia, Europe and other countries
- Tel: +44 (0)1869 243 241
- Antibodies: antibodies.eu@inotiv.com
- Bioproducts: biologicals.eu@inotiv.com

Monoclonal Antibody Production

Customer experiences

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